Background: Aphis gossypii Glover is among the most economically important sap-feeding insect pests affecting vegetable, fruit, fibre, and ornamental crops worldwide. Heavy infestations reduce plant vigour through direct feeding while also transmitting numerous plant viruses. Increasing resistance to synthetic insecticides and concerns regarding environmental contamination have encouraged the exploration of environmentally sustainable biological control agents. Entomopathogenic fungi have emerged as promising alternatives because they infect insects naturally without requiring ingestion and are generally compatible with integrated pest management strategies.

Objective: This educational manuscript aimed to demonstrate the preparation of a scientific research article by evaluating the simulated efficacy of three entomopathogenic fungal treatments against Aphis gossypii under controlled greenhouse conditions.

Method: A completely randomized greenhouse experiment was simulated using four treatment groups comprising an untreated control and three fungal formulations. Each treatment consisted of five replicates containing twenty aphids. Simulated observations included aphid mortality, colony growth rate, leaf damage index, and fungal infection percentage. Statistical analyses included descriptive statistics, one-way analysis of variance, and Tukey's Honestly Significant Difference test.

Results: The simulated dataset demonstrated significant reductions in aphid population growth and leaf damage following fungal application. Treatment F₃ produced the highest mortality and fungal infection percentage while substantially reducing colony development compared with the untreated control.